Molecular identification of house fly, Musca domestica L. (Diptera : Muscudae), using mitochondrial DNA partial genes cytochrome oxidase sub unit 1 (CO1) in Manado city

Abstract

Musca domestica L. becomes a serious problem in tropical country. Its role as a vector of many pathogenic microbes has caused many health problems for humans. A study was conducted to identify house fly in Manado City, using partial gene cytochrome oxidase sub unit 1 (CO1). House fly is obtained from nine different habitats in Manado City. Isolation of DNA were used DNA extraction and purification Kit. Amplification of CO1 gene by PCR method. Sequence analysis using Geneous and MEGA 6.0. The result of this research showed, the sequence of house fly CO1 gene : IBP, IBS, IBT, IKT, IMT and IPB have the highest similarity level with Musca domestica cytochrome oxidase subunit I (COI) gene [MG557665.1], while the CO1 gene of IKP and IKT has the highest similarity level with Musca domestica ISOLATE CSU 140601CBJI A $ cytochrome oxidase subunit I (COI) gene [KY001857.1]. CO1 gene of IKS showed similarity with Musca domestica ISOLATE CSU 140601CBJI A$ cytochrome oxidase subunit I (COI) gene. Intraspecies genetic variation of house flies in Manado city based on partial CO1 gene, are high.